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KMID : 0350519960490020735
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Journal of Catholic Medical College
1996 Volume.49 No. 2 p.735 ~ p.743
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Effect of Partial Bladder Outlet Obstruction on Bladder Muscle Collagen Content and contractility in Rabbit
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Abstract
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Vesical outlet obstruction by prostate hypertrophy produce morphological, biochemical and functional changes. Partial obstruction of the bladder outlet induces a rapid hypertrophy characterized by increased bladder mass and collagen deposition in
smooth
muscle cell and reduced contractile response of the bladder to electrical and pharmacological stimulation. Collagen fibrils in smooth muscle probably have an important role in intercellular transmission of active force and the changed collagen
concentration in bladder muscle might thus also affect the contractile properties of the smooth muscle, but it is not well known that the precise mechanisms of decreased bladder muscle contractility. And it has not been studied whether
correlation
according to the duration of partial bladder outlet obstruction. This study was designed to determine the effect of partial bladder outlet obstruction on bladder muscle contractility and collagen content and whether the reduced bladder muscle
contractility was related to increased to increased collagen content in partially obstructed rabbit urinary bladder. The contractile response was recorded on a Grass 7D polygraph via an FT. 03 force transducer and expressed as the g tension per
100mg.
Total collagen content and collagen concentration in hypertrophied bladder muscle were measured quantitatively according to the duration of obstruction. The amount of collagen was calculated from its content of hydroxyproline by assuming that
hydroxyproline is 14% in weight of the collagen.
@ES The results were as follows:
@EN 1. During the 4 weeks of obstruction the detrusor weight progressively increased (P<0.05).
2. Contractile response to field stimulation (2, 4, 8, 16, 32 Hz) and bethanechol (2.5×10E-4 M) were gradually reduced by increasing duration of partial outlet obstruction (P<0.05).
3. The total amount of detrusor collagen were significantly higher than that of control (105.9±5.6mg) (P<0.05). It was measured in 1, 2, 3 and 4 weeks of obstruction to be 131.2±7.5, 552.9±22.5, 752.5±0.9 and 680±21.3mg, respectively. The
collagen concentration (per 50mg of bladder tissue) was tends to be decreased in 1 week of obstruction (1551.3±88.1㎍) compare to control (1924.8±101.9㎍), but increased in value in 2, 3 and 4 weeks of obstruction. It was measured
2792.3±113.7,
3051.6±84.7 and 2181.7±68.2㎍, respectively (P<0.05).
4. It was revealed that the significant negative correlation was shown statistically between collagen content and bladder muscle contractility by regression analysis for the effect of collagen contren on bladder muscle contractility (P<0.05).
Conclusively the bladder outlet obstruction cause decrease of detursor muscle contractility gradually according to the duration of obstruction which was correlated to progressive increase of total collagen and collagen concentration.
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